Virus:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Plasmid Preparation:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Bioprocessing:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Recombinant:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Purification:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
RNAscope:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Multiplex Assay:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Immunohistochemistry:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
Staining:
Article Title: Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-phospho ribosomal protein 6 pSer244/pSer247 Invitrogen Cat#44-923G; RRID: AB_2533798 Chicken anti-GFP Abcam Cat#ab13970; RRID: AB_300798 Chicken anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit anti-cfos Cell Signaling Cat2250S; RRID: AB_2247211 Goat anti-rabbit IgG Alexa 488 Invitrogen Cat#A11008; RRID: AB_143165 Goat anti-rabbit IgG Alexa 594 Invitrogen Cat#A11072; RRID: AB_142057 Goat anti-chicken IgG Alexa 488 Invitrogen Cat#A11039; RRID: AB_142924 Goat anti-chicken IgG Alexa 594 Invitrogen Cat#A11042; RRID: AB_142083 Rabbit Anti-PKC delta Abcam Cat#ab182126 Goat anti-rabbit IgG Alexa 647 Invitrogen Cat#A21245; RRID: AB_2535813 Bacterial and virus strains AAV-FLEX-GFP Addgene Cat#28304; RRID: Addgene_28304 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat#44361; RRID: Addgene_44361 AAV-hSyn-DIO-mCherry Addgene Cat#50459; RRID: Addgene_50459 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Addgene Cat#44362; RRID: Addgene_44362 AAV5-CaMKIIa-hM3D(Gq)-mCherry UNC Vector Core N/A AAV5-CaMKIIa-hM4D(Gi)-mCherry UNC Vector Core N/A AAV5-hSynapsin1-FLEX-axon-GCaMP6s Addgene Cat#112010; RRID: Addgene_112010 H129DTK-TT Lo and Anderson, 2011 N/A retroAAV-CAG-GFP Addgene Cat#37825-AAVrg; RRID: Addgene_37825 AAV1-phSyn1(S)-FLEX-tdTomato-T2ASypEGFP-WPRE (Synaptophysin) Salk N/A CAV-GFP Montpelier N/A pAAV-Ef1a-DO_DIO-TdTomato_EGFPWPRE-pA (color flipping) Addgene Cat#37120-AAVrg; RRID: Addgene_37120 AAVrg-CAG-FLEX-tdTomato Addgene Cat#28306-AAVrg; RRID: Addgene_28306 AAV5-CAMKIIa-mCherry UNC Vector Core N/A pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene Cat#100845; RRID: Addgene_100845 pAAV-FLEX-ArchT-tdTomato Addgene Cat#28305; RRID: Addgene_28305 AAV-pgk-Cre Addgene Cat#24593; RRID: Addgene_24593 pAAV-syn-FLEX-splitTVA-EGFP-tTA Addgene Cat#100798; RRID: Addgene_100798 pAAV-TREtight-mTagBFP2-B19G Addgene Cat#100799; RRID: Addgene_100799 G-deleted Rabies Salk Viral Vector Core N/A Chemicals, peptides, and recombinant proteins Clozapine-N-Oxide Tocris Bioscience Cat#4936; CAS:34233-69-7 CCK Tocris Bioscience Cat#1166 Critical commercial assays Agilent Absolutely RNA Nanoprep Kit Agilent Cat#400753 RNAeasy Mini kit Qiagen Cat#74104 RNAscope Fluorescent Multiplex Kit Advanced Cell Diagnostics (ACD) Cat# 320850 RNAscope Probe-Nos1 ACD Cat#437691 RNAscope Probe-mCherry ACD Cat#431202 RNAscope Probe-Vglut1 ACD Cat#416631 RNAscope Probe-Vgat ACD Cat#319191 (Continued on next page) Cell Metabolism 33, 1418–1432.e1–e6, June 6, 2021 e1
Article Title: Nausea-induced suppression of feeding is mediated by central amygdala Dlk1-expressing neurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-beta Galactosidase (anti-LacZ) Abcam Cat#ab9361; RRID: AB_307210 Chicken polyclonal anti-GFP Thermofischer scientific Cat#A10262; RRID: AB_2534023 Rabbit anti-cFos Cell Signaling Technology Cat#2250; RRID: AB_2247211 Goat polyclonal anti-CGRP Abcam Ca# ab36001; RRID: AB_725807 Rabbit anti-calretinin (Calb2) swant Ca#CR7697; RRID: AB_2619710 Mouse anti-VGAT Synaptic Systems Ca#131011; RRID: AB_887869 Alexa Fluor 488 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Alexa Fluor 488 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-545-003; RRID: AB_2340428 Alexa Fluor 488 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-545-155; RRID: AB_2340375 Cy3 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-165-147; RRID: AB_2307351 Cy3 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-165-152; RRID: AB_2307443 Cy3 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-165-155; RRID: AB_2340363 Alexa Fluor 647 Donkey Anti-Chicken Jackson ImmunoResearch Cat#703-605-155; RRID: AB_2340379 Alexa Fluor 647 Donkey Anti-Goat Jackson ImmunoResearch Cat#705-605-147; RRID: AB_2340437 Alexa Fluor 647 Donkey Anti-Rabbit Jackson ImmunoResearch Cat#711-605-152; RRID: AB_2492288 Bacterial and virus strains AAV9-pAAV-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al.51 Addgene 44361-AAV9 AAV9-pAAV-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al.51 Addgene 44362-AAV9 AAV2-pAAV-hSyn-DIO-mCherry Bryan Roth Addgene 50459-AAV2 AAV5-Ef1a-DIO-ChR2-EYFP Mattis et al.52 Addgene 35509-AAV5 AAV5-Ef1a-DIO-EGFP Karl Deisseroth Addgene 27056-AAV5 AAV5-EF1a-DIO-eArch3.0-EYFP Karl Deisseroth UNC vector core AAV5-EF1a-fDIO-hChR2(H134R)-EYFP-WPRE Karl Deisseroth UNC vector core AAV-synP-DIO-STPEPB Ian Wickersham UNC vector core EnvA G-deleted rabies Karl-Klaus Conzelmann Gene Center Munich, LMU Chemicals, peptides, and recombinant proteins Triton X-100 CarlRoth Cat#3051 NaCl 0.9% Braun Cat#21222452 Clozapine-n-oxide (CNO) Sigma-Aldrich Cat#C0832-5MG Isoflurane Cp-pharma AP/DRUGS/220/96 Rimadyl (carprofen) Zoetis Cat#1939541 (Continued on next page) Cell Reports 43, 113990, April 23, 2024 17
Article Title: Defensive Behaviors Driven by a Hypothalamic-Ventral Midbrain Circuit
Article Snippet: Brain sections used for IHC were stained with the following primary antibodies, followed by secondary antibodies: mouse anti-tyrosine hydroxylase (TH; Millipore, MAB318)/Alexa Fluor 488, donkey anti-mouse or Alexa Fluor 594 donkey anti-mouse; rabbit anti-cFos (Cell Signal #2250)/Alexa Fluor 488 donkey anti-rabbit, or Alexa Fluor 594 donkey anti-rabbit.
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